About the Department

History

  • Agricultural Biochemistry research was initiated in 1970 as a small Biochemistry unit under the Department of Crop Physiology and Biochemistry in U. P. Institute of Agricultural Sciences, Kanpur. The teaching of Plant Biochemistry started in 1972 as a course for the award of M.Sc. (Ag.) degree in Plant Physiology. With the establishment of C. S. Azad University of Agriculture and Technology on March 1, 1975 and subsequent introduction of semester system in the year 1976, the core and elective courses in Plant Biochemistry were introduced at U. G. level.
  • A separate and independent Department of Agricultural Biochemistry came into existence in the year 1983. Soon after, in 1984, a four semester course for the award of M.Sc. (Ag.) degree in Agricultural Biochemistry was started. Ph.D. programme in Agricultural Biochemistry commenced from 1986. Since then the faculty members of this department are actively associated with the teaching, research and extension activities of the University.

Mission

  • Despite national food security in terms of total food production sufficient for per capita supply of food, nearly 208 million people suffer from hidden-hunger caused due to deficiency of protein, vitamin A, iron, iodine, zinc etc. Industrial use and export of food crop is also limited due to lack of suitable varieties for specific end-use. Thrust of future research has been laid upon health related aspects of agriculture with a focus on the development of crop varieties with enhanced nutritive value, processing qualities and market value.

Mandates

  • To generate specialized manpower in the discipline of Agricultural Biochemistry.
  • To render necessary support for improvement of crops at the University level and in development of food crop varieties having superior quality traits in respect of nutritive value and industrial use.
  • To develop techniques for better utilization of waste/ underutilized resources viz. green biomass and oilseed cakes etc. as feed or food.

About the faculty of the Department

Employee ID
:
CSAM068
Name
:
Dr.Madhu Vajpeyi
Department
:
Agricultural Biochemistry
Designation
:
Professor &Head (Agrl. Biochem.)
E-mail
:
vajpeyimadhu@yahoo.co.in
E-mail2
:
agricultural_biochemistry@rediffmail.com
Mobile No.
:
09936603377
Qualification
:
M.Sc.(Organic Chemistry),Ph.D.(Agrl. Biochemistry)
Key Area/Specialization
:
Quality of Oilseeds  and their value added products ,wheat grain quality,
Employee ID
:
CSAM047
Name
:
Dr.Mukesh Mohan
Department
:
Agricultural Biochemistry
Designation
:
Professor
E-mail
:
drmukeshmohan@rediffmail.com
E-mail2
:
drmukeshmohan@gmail.com
Mobile No.
:
07897362855
Qualification
:
M.Sc.(Agrl. Biochemistry),Ph.D.(Agrl. Biochemistry)
Key Area/Specialization
:
Foodgrain quality,leaf nutrient extraction,nutraceutical potential of chloroplast
Employee ID
:
CSAL011
Name
:
Dr.Lallu Singh
Department
:
Agricultural Biochemistry
Designation
:
 Professor
E-mail
:
Lallu1960@yahoo.com
E-mail2
:
Mobile No.
:
9450154804
Qualification
:
M.Sc.(Ag) in Agrl. Chemistry with specialization in Agricultural Biochemistry,D.Fil  in Sciences ( Agrl. Biochemistry )
Key Area/Specialization
:
Nutritional Quality of some oil seeds and cereals.
Employee ID
:
CSAR145
Name
:
Dr.Rakesh Babu
Department
:
Agricultural Biochemistry
Designation
:
Assistant Professor
E-mail
:
rakeshbabugautam@yahoo.co.in
E-mail2
:
Mobile No.
:
09450141699
Qualification
:
M.Sc.(Agrl. Biochemistry),Ph.D.( Agrl. Biochemistry)
Key Area/Specialization
:
Biochemistry of Food Legumes.
Employee ID
:
CSAN 007
Name
:
Dr.Nand Kumar
Department
:
Agricultural Biochemistry
Designation
:
Assistant Professor/Assistant Biochemist(Rice)
E-mail
:
kumarnand512@yahoo.co.in
E-mail2
:
Mobile No.
:
09415441077
Qualification
:
M.Sc.(Agrl. Biochemistry),Ph.D.( Agrl. Biochemistry)
Key Area/Specialization
:
Rice Quality

Information of Officers

S.N. Name of teacher/scientist Designation Mobile No. Email I.D.
1. Dr.Madhu Vajpeyi Professor/HOD(Agrl. Biochem.) 09936603377 vajpeyimadhu@yahoo.co.in
2. Dr.Mukesh Mohan Professor 07897362855 drmukeshmohan@rediffmail.com, drmukeshmohan@gmail.com
3. Dr.Lallu Singh Professor 09450154804 lallu1960@gmail.com
4. Dr.Rakesh Babu Assistant Professor 09450141699 rakeshbabugautam@yahoo.co.in
5. Dr.Nand Kumar Assistant Professor 09415441077 kumarnand512@yahoo.co.in

Organogram of the Department

S.N. Name Designation Work Allotted Work Place Telephone/Mobile E-mail
1. Dr.Madhu Vajpeyi Professor/HOD(Agrl. Biochem.) Teaching & Guiding M.Sc. & Ph.D.students, /Research, Administration of the department Dept.of Agrl. Biochemistry 09936603377 vajpeyimadhu@yahoo.co.in
2. Dr.Mukesh Mohan Professor Teaching & Guiding M. Sc. & Ph.D.students of the department Dept.of Agrl. Biochemistry 07897362855 drmukeshmohan@rediffmail.com, drmukeshmohan@gmail.com
3. Dr.Lallu Singh Professor Teaching UG & PG & Ph.D.students , Guiding M.Sc. students of the department Dept.of Agrl. Biochemistry 09450154804 lallu1960@gmail.com
4. Dr.Rakesh Babu Assistant Professor Teaching UG & PG & Ph.D.students , Guiding M.Sc. students of the department Dept.of Agrl. Biochemistry 09450141699 rakeshbabugautam@yahoo.co.in
5. Dr.Nand Kumar Assistant Professor Teaching UG & PG & Ph.D.students , Guiding M.Sc. students of the department Dept.of Agrl. Biochemistry 09415441077 kumarnand512@yahoo.co.in
6. Sri Ashok Kumar Singh Lab assistant To assist in conducting practicals of UG/PG UG/PG Labs of Dept.of Agrl. Biochemistry 9415477711
7. Sri Anil Kumar Mishra Head Clerk To look after store,audit,administration ,budget,Incometax&superwision of the work of office Dept.of Agrl. Biochemistry 9956622065
8. Smt. Nisha Srivastava Junior Clerk Typing work,G.P.F.,G.I.S.,Salary,Despatch ,Audit And work related to students Dept.of Agrl. Biochemistry 7897695297
9. Sri Gangesh Kumar Shukla Junior Clerk Attached to Security Office Security Office 9415772201
10. Sri Jai Narain Pandey Junior Clerk Work related to salary Dept.of Agrl. Biochemistry 8858641777
11. Smt. Geeta Singh Lab Attendant To mentain the chambers of Dr.Er. P. K. Bisaria,Dr. Nand Kumar and Class Room Dept.of Agrl. Biochemistry 9450333529
12. Sri Rampher Lab Attendant To open and Close the department and to distribute letters Dept.of Agrl. Biochemistry 7318535561
13. Sri JaiNarain Peon To distribute letters and other works of office Dept.of Agrl. Biochemistry 7523830340
14. Smt. Ram Dulari Temporary Attendant To mentain the chambers of Dr. Lallu Singh,Dr. Mukesh Mohan and to distribute letters and other works of office as and when needed Dept.of Agrl. Biochemistry 9696746858
  • Rules /Sub Rules/Work Policy,if any:Governed by University Act & Statutes
  • Details of programmes /services/schemes run by department: NIL
  • Departmental Policies and financial sanction related GO’s: No separate policy, Governed by university ‘s policies.
  • Seniority list of officers/ teachers/technical staff of the department:
S.N. Name Designation
Teachers
1. Dr.Madhu Vajpeyi Professor/HOD(Agrl. Biochem.)
2. Dr.Mukesh Mohan Professor
3. Dr.Lallu Singh Professor
4. Dr.Rakesh Babu Assistant Professor
5. Dr.Nand Kumar Assistant Professor
Non-Technical Staff
7. Sri Ashok Kumar Singh Lab assistant
8. Sri Anil Kumar Mishra Head Clerk
9. Smt. Nisha Srivastava Junior Clerk
10. Sri Gangesh Kumar Shukla Junior Clerk
11. Sri Jai Narain Pandey Junior Clerk
12. Smt. Geeta Singh Lab Attendant
13. Sri Rampher Lab Attendant
14. Sri JaiNarain Peon
15. Smt. Ram Dulari Temporary Attendant
  • Success Stories 
  • Salient achievements

Teaching

  • The Department is regularly running P.G. programme leading to the award of M.Sc.(Ag.) and Ph.D degrees in Agricultural. Biochemistry.
  • Every year 6 student in M.Sc.(Ag.) and 2 students in Ph.D. programme are admitted in the Department.
  • M.Sc.(Ag) and Ph.D Students are required to conduct research and submit thesis in the partial fulfillment of the award of respective degrees.
  • The faculty members of the Department have been teaching Biochemistry course to B.Sc.(Agriculture), B.Sc.(Forestry) and B.Sc. (Horticulture) and B.Sc.(Home Science )students of the university.
  • In last five years, 10 students at M.Sc.(Ag) level and 3 students at Ph.D level have been awarded respective degrees in Agricultural Biochemistry by the University.
  • Department is offering revised courses suggested by Dean’s committee constituted by ICAR.

Courses offered in U.G. and P.G. Programmes 

B. Sc. Hons in Agriculture. ,B.Sc.Hons. in Forestry,B.Sc.Hons. in Horticulture
Course No. Course Title Credit Hrs. Semester
ABC-301 Biochemistry 3(2+1) III
BSH-101
ABC-101
Plant Biochemistry and Biotechnology
Plant Biochemistry and Biotechnology
3(2+1)
3(2+1)
I
I
M.Sc. (Ag.)
Course No. Course Title Credit Hrs. Semester
ABC-501 Basic Biochemistry 4 (3+1) I
ABC-502 Intermediary Metabolism 3 (3+0) I
ABC-503 Enzymology 3 (2+1) II
ABC-504 Molecular Biology 3 (2+1) III
ABC-505 Techniques in Biochemistry 3 (1+2) II
ABC-507 Plant Biochemistry 3 (3+0) II
ABC-509 Food and Nutritional Biochemistry 3 (2+1) III
ABC-599 Course Seminar 3 (3+0) IV
Ph. D. Programme
Course No. Course Title Credit Hrs. Semester
ABC-601 Biochemistry of Nucleic Acids and Proteins 3 (2+1) I
ABC-603 Biochemistry of Crop Quality 4 (3+1) I
ABC-602 Immunochemistry 2 (2+0) II
ABC-604 Agricultural Biotechnology 3 (3+0) II
ABC-605 Biochemistry of Biotic and Abiotic Stresses 3 (3+0) III
ABC-699 Course Seminar 1 III

Research
Crop quality improvement programme

  • Identification of Genotypes Superior in Quality Traits
Toria High oil Low erusic acid Low glucosinolate ORT(M), 5-2, MCT 96, BAUST 4, BAUST 1(43-44%)21-2, 21-3, 21-9, ( 3 to 10%)22 SPS
Rai mustard High oil PSR 29, RK 9807, AD 2041, SEJ 2, JGM 97-82 (43-46%)
Sesame High oil JT 58, TC 25 (56%)
Linseed High oil LMH 90-7, NL 115, SLS 24, LCK 9313, LCK 9708, LCK 9801, 12 X 45 (45-46%)
Low linolenic acid LCK 9319 (32%)
  • Development of crop varieties with Improved Quality traits.
Crop Trait Varieties
Wheat High protein Gangotri , Deva, Halna, Ujiyar (13-14% protein)
Linseed High oil Sheela (41% oil, yield 1.3-1.4 q/ha) Shekhar (43% oil, yield-1.5-1.6 q/ha) Rashmi (41% oil) (fibre-7q/ha, yield 1.0q/ha)
Rai mustard (B. juncea) High oil Varuna, Rohini, Vaibhav, Verdan, Urvashi, Basanti, Kanti, Maya ( oil content 40-43%).
Toria (B.compestris) High oil Type-9, Bhawani (44- 45% oil) Type-151 (45- 47% oil)

Achievements in specific trials: 

  • A scented sorghum landrace (accession No. MASC-1/2002), collected from Bundelkhand region of Uttar Pradesh, possessing basmati rice like aroma has been identified. Ear heads and immature seeds (dough stage) of this land race emit mild scent and are used in culinary preparations. This novel cultivar has been conserved in National gene bank/ NBPGR (Id. no. IC546941) and may be utilized for transfer of aroma trait to other selected sorghum cultivars.
  • A high protein landrace (accession No. MASC-5/2002) of grain sorghum, locally known as “Pola” in Hamirpur district of Uttar Pradesh, possessing 14.34% protein was identified.Utilization of this high protein cultivar may be advantageous for improvement of seed protein content of sorghum varieties and, thus can ensure nutritional security of poor people who subsist on sorghum as staple food.
  • Azad Basmati, a high yielding variety of basmati rice released by the University, turned out to be best in respect of Head rice recovery (62%) and Kernel elongation ratio on cooking (2.2) as compared with check varieties( HRR – 42-49%, KER- 1.38-2.03). Kernel-length(1.6mm) and L/B ratio(3.9) of Azad basmati were comparable to most check varieties but fell marginally short than the desired minimum kernel- length (6.6mm) for basmati rice for export purpose.
  • Alarmingly high amounts of oxalates in Bathua (Chenopodium album L.) – A health concern for kidney patients: Bathua (tender shoots of C.album L.), a hand- picked winter weed from rabi crops, is a popular leafy–vegetable widely consumed in various forms throughout the country especially in North India. Investigations carried out on food value and status of anti-nutritional substances revealed that Bathua, although a rich source of protein (35.42%, dwb), Pro-vitamin A carotenoids, macro- and micro-elements, contains alarmingly high amount of harmful oxalates (18.22% dwb) and phenolic substances (3.48%, dwb).
  • Nutraceutical potential of chloroplast isolated from Fenugreek (Trigonella foenum graecum L.) leaves: Freeze dried chloroplast isolated from leafy Fenugreek was found to be a high-density nutrient concentrate having a unique blend of nutrients that no single natural food can provide. The value added product possessed 13.4 fold protein,126x lipids, 3x total minerals, 18x K, 115x Fe, 11x Mn, 32x Cu and 184x carotenoids than in green fenugreek leaves.

The isolated chloroplast preparation has higher calorific value (1.3x), more fat (3x) possessing 80% unsaturated fatty acid, Fe (6x), K (550x) and carotenoids (1.43x) as compared to Spirulina. Further, caffeic acid (40.45 mg /100g) and protocatechuic acid (11.36mg /100g) present in the chloroplast preparation are reported to have antioxidant value. It, thus, may be concluded that freeze dried chloroplast preparation from fenugreek greens, having excellent blend of nutrients may find enormous nutraceutical applications.

  • Evaluation of breeding material of rapeseed-mustard for oil content:
    Among rapeseed–mustard genotypes /varieties tested for oil content, the genotypes MK(E)-103,MK-11-107(>41%)in B.juncea, TK-9-2,TK-7-14(>44%)in B.rapa var.Toria and YSKM-11-202AND YSKM -11-107(>44%)were identified as high oil containing genotypes. RKE-9-1(43.07), RKE-8-2(42.89) in B.juncea, TKM-12-105 (45.29),TK- 9-1 (45.23), TK -9-3 (45.14), TKM-12-101 (44.96), TKM-12-106 (44.75) in B.rapa var.Toria and YSKM-12-106(45.36), YSKM-10-2(44.98)were identified as high oil containing genotypes.
  • Evaluation of oil quality of rapeseed-mustard from Advance Varietal Trial:
    Among rapeseed –Mustard genotypes /varieties tested for fatty acid composition, LES -43 and LES-45 containing 4.05and2.75% erucic acid and Pusa mustard-21(1.81%) and LES-44(1.25%)were identified as low erucic material suitable for quality breeding as these contained less than 2% erucic acid in oil..
  • Anti-nutritional and antioxidative factors in seed meal of Brassica entries :
    Out of 50 Brassica entries tested for Vitamin C and polyphenol content in their defatted seeds (meal), Vitamin C content was found to vary from 78.9 to134.6 mg/100g, the genotypes namely Bio -43, and Bio-45 recorded appreciably higher content of this anti-oxidant. As regards polyphenol content, it varied from 1.525 to 2.561%, the lowest and the highest values being given by the genotypes Bio- 37and Bio-27.
  • Processing techniques for better utilization of Bathua leaves:
    Investigations carried out to improve utilization of bathua through processing revealed that blanching of fresh bathua foliage in boiling water resulted in loss of total solid(2.83to 24.91%), nutritionally important microelements namely Zn(16.98to 43% ),Cu( 4.36to 38.09%) and Mn( 34.69 to 60.20%).
  • Biochemical evaluation of some varieties/hybrids of baby corn grown in kharif season:
    Among the varieties/hybrids of baby corn, hybrid R-2005-5 recorded highest amount of dry matter(9.82%),phosphorus(68mg/100g) and protein(1.05%) as compared to others.
  • Blending mustard oil with other edible oils to identify the blend best suited for edible purpose:
    Oils and fats are essential nutrients which perform various functions in our body. None of the existing oils perfectly matches the WHO recommendations of a healthy cooking oil (SFA: MUFA: PUFA =1:1-3:1) This was only possible by blending judicious amount of the existing edible oils hence sesame and groundnut oils were chosen for blending with mustard oil. Among the oil-blends, mustard: sesame(30:70) and mustard: groundnut(30:70) proved to be the best from edible point of view whereas the oil of the blend mustard: groundnut(30:70) was found to be the most stable one as it showed the highest value of MUFA:PUFA
  • Effect of delayed sowing on biochemical composition of seeds of some mustard varieties/genotypes:
    In mustard, due to delay in sowing, from 15 Oct to 23 Nov, a decrease in 1000 seed weight and oil content was observed while protein content was found to increase. Sowing at optimum time recorded highest 1000 seed weight. Among varieties, highest 1000 seed weight was recorded by the genotype NRCDR -02 (4.57 g). Among the interactions, the genotype BPR-540-6 at first date of sowing (15 Oct), recorded highest 1000 seed weight (5.62 g) while at second date of sowing (23 Nov.) NRCDR-02 recorded highest test weight (4.11 g). Among the varieties, NPJ-113 recorded highest oil content, this genotype also performed the best in both the sowing dates. However NPJ-113 recorded lowest protein content among the varieties and also at first date of sowing.
  • Variability in quality parameters of desi & Kabuli type chickpea varieties:
    The range of variability in Kabuli chickpea varieties were from 61.70-65.03% (Total carbohydrates), 23.38 – 25.90 % (Crude Protein), 4.12- 5.03% (Crude fat) and 373.48 – 407. 00 K cal/100g (calorific value) however in case of desi chickpea varieties, the rang of variability was as follow in total carbohydrates from 51.74- 65.05 % in crude protein from 23.68 – 25.57%, in crude fat from 3.62 – 4.62 % and in calorific value from 345.19 – 385.57 k cal/100g.Among the Kabuli & desi chickpea varieties tested, variety- BG 1053 (Kabuli type) appeared superior most from nutritional point of view while in case of desi chickpea varieties- Vishal & Sadabahar showed higher value of crude protein, calorific value, crude fat & total carbohydrates.
  • Effect of variance in dietary bulk nutrients and Ayurvedic/ Allopathic medicinal supplement on the blood glucose profiles of pre-diabetic /Type-2 diabetic subjects:
    During the study, it was found that a higher dietary protein and fibre go to reduce Post Prandial Blood Glucose (PPBG) level carbohydrates increased them. Among the allopathic medicines ‘Metformin’ reduced the mean value of fasting blood glucose (FBG) from 199 mg/dl to 130 mg/dl and PPBG form 270 mg/dl to 142 mg/dl on consuming one pill per day for a Type-2 diabetic person.

Other Activities of the Department of Agricultural Biochemistry

  • The Dept. is the HQ of Indian Society of Agricultural Biochemists and Society for Green Vegetation Research. Indian Journal of Agricultural Biochemistry, a biannual Journal, is being published regularly since 1988.
  • National Symposium on Biochemistry of Photosynthesis & Nitrogen Assimilation, Oct., 27-28, 1988.
  • Second convention of the Society for Green Vegetation Research, Oct. 28-29, 1988.
  • International conference on Biotechnology in Agriculture and Forestry, Feb., 15-18, 1993, in collaboration with IARI, New Delhi.
  • DBT sponsored Popular Lecture Series on Biotechnology, 1994.
  • ICAR Summer School on Recent Advances in Quality of Cereals, Pulses & Oilseeds, May 24 to June 14, 1999.
  • National Symposia on Regulation of Metabolic Pathways for Crop Improvement and Recent Trends in Green Vegetation & Leaf Protein Research, Feb., 24-26, 2000.
  • The Department successfully organized An International Conference on Post Harvest Technology and Value Addition of Cereals, Pulses and Oilseeds on Nov. 27-30, 2006. tne proceeding of the conference are been published shortly.

  • Budget allocation of different plans/schemes: No separate plan or scheme is running in the department
  • Details of departmental activities beneficial for the farmers: The details are given below:
  • A domestic technique to reduce harmful oxalates and phenolic substances from Bathua (Chenopodium albumL.) Considering high amounts of oxalates the benefits of eating Bathua may out weight the risks especially to those suffering from hypocalcaemia and/or recurrent kidney stone problems. A simple technique that housewives can adopt to remove harmful substances from Bathua has been developed.
    Blanching of Bathua foliage in boiling water for 5 minutes followed by rinsing the blanched mass in cold water removed up to 76% of the oxalates and 59% phenolic substances. Treated Bathua contained higher protein (42.43%) but lower oxalates (4.4%, dwb) and phenolics (1.43%, dwb) than in unprocessed Bathua. Increasing blanching time to 10 minutes could further enhance removal of oxalates and phenolic substances up to 80% and 72%, respectively, however resulted in greater loss of minerals (42%).

Effect of processing on nutritive value and harmful substances in Bathua (g/100g, dwb):

Treatment Crude Protein Total ash
(minerals)
Total Oxalates
(minerals)
Total Phenolic substances
(minerals)
No treatment (control) 35.42 19.01 18.22 3.48
Water Blanching (5 min.) +Rinsing 42.43 (+19.79) 12.10 (-36.35) 4.40 (-75.85) 1.43 (-58.95)
Water Blanching (10 Min.) +Rinsing 43. 21 (+21.99) 11.08 (-41.71) 3.65 (-79.79) 0.97 (-72.06)

Figures in parenthesis denote % increase (+) or decrease (-) in the treatment as compared to control

  • Better Utilization of surplus Bathua through fractionation: Since large quantity of bathua foliage is left unutilized in the fields where it is hand picked as weed, effort were made to extract leaf protein for food using crop fractionation process. Bathua foliage being rich in protein was found to be suitable for leaf-protein extraction, yielding 36g Leaf Protein Concentrate (LPC per kg fresh foliage) containing 53.3% protein, 16.4% ether extractives, 11% mineral matter and 19.4% NFE. Fractionation of Bathua proved advantageous not only for utilization of the bulk that otherwise goes waste but also for reduction of undesirable substances present in it. Fractionation for LPC extraction removed 71% oxalates, 81% phenolics and 96% fibres of bathua.

Distribution of oxalates and phenolic substances in fractionation products of Bathua (as % of total contents in Bathua):

Bathua Fractions Total Oxalates  Phenolic Substances
Unprocessed Bathua 100 100
Pressed Fibrous Residue 35 40
Extracted Juice 65 60
LPC 29 19

Carefully prepared, acid washed Bathua LPC containing 61% protein, 4% oxalates and 0.9% phenolics was more nutritious than unprocessed Bathua LPC.

Effect of washing of wet LPC (pH 4.0) on the contents of protein, oxalates and phenolics in dry LPC:

Treatment Crude Protein Total Oxalates Phenolic Substances
Unwashed LPC (Control) 56.65 12.18 1.27
Once washed LPC 6.92 (+7.54) 7.23 (-40.64) 1.15 (-9.45)
Twice washed LPC 61.52 (+8.60) 4.04 (-66.82) 0.93 (-26.77)

Figures in parenthesis denote % increase (+) or decrease (-) in the treatment over control

  • Utilization of vegetable crop residues through fractionation: Certain vegetable crops also give an abundance of byproduct foliage besides main product, which has been utilized for extraction of protein for food/ feed use. Potato, carrot and cauliflower crops yield 40, 22 and 13q byproduct foliage/ ha, fractionation of which produced 835, 265 and 190kg LPC/ha, containing38, 33 and 55% protein, respectively. Protein carotene-xanthophyll rich LPC has been successfully used to replace GNC in poultry ration.
  • Detoxication of linseed (Linum usitatissimum L.) meal for enrichment of wheat flour Chapati: Linseed meal is rich in protein having fairly high digestibility and biological value but its use as a food is hampered due to the presence of a cyanogenic glycoside ‘Linamarin’. A simple technique for detoxification of linseed meal to enable its use as food supplement was developed. Incubation of linseed meal at 50% moisture regime for 30 minutes at room temperature removed most of the cyanogens (99%) from linseed meal.

Effect of Moisture regime and incubation period on the cyanogens in linseed meal:

Incubation period (min) Moisture regimes (%) Cyanogens (g HCN/ 100 g meal )
Control 0 0.480
30 50 0.004 (-99.17)

Figures in parenthesis denote % decrease (-) in the treatment over control

Chapaties prepared from Wheat flour (WF) – Detoxified linseed meal (DLM) blends in different ratios (95:5, 90:10, 85:15, 80:20, 75:25 and 70:30, w/w) were evaluated for organoleptic attributes. Organoleptically acceptable chapati having 61% higher protein than in the wheat flour chapati could be prepared from WF – DLM blend in 80:20(w/w) ratio.

  • Reports of departmental programmes /schemes:  NA
  • Important statistical data related to department:  NA
  • Proceedings of meetings held in the department:
  • Proceedings of Research Advisory Committee ,Meeting –Rabi-2014-15

Research Advisory Committee ,Meeting for Rabi-2014-15 of the department, chaired by The Director Research, was held on 01.010.2014.The following faculty attended the meeting:

1 Dr.M.P.Yadav Director Research
2 Dr Madhu Vajpeyi I/C
3 Dr Mukesh Mohan Assoc.Prof , Agri Biochem
4 Dr Anil Kumar Singh Assis Prof
5 Dr AK Sachan Assist Director Seed& Farm
6 Dr Rakesh Babu Assist Prof Biochem
7 Dr SB Pandey Assist Prof Soil Sci
8 Dr Lallu Singh Assoc .ProfAgri Biochem
9 Dr S K Singh Assis Prof
10 Dr Neerja Agarwal I/C Entomology
11 Dr Rajendra Prasad HOD Plant Pathology

Results of previous years experiments were displayed and discussed. New programs on biochemical aspects of some crops were discussed and finalized . All the programs were approved as such.

  • Proceedings of Research Advisory Committee ,Meeting Rabi-2016-17

Research Advisory Committee ,Meeting for Rabi-2016-17 of the department, chaired by The Director Research, was held on 08.11.2016.The following faculty attended the meeting:

1 Dr.Hargyan Prakash Director Research
2 Dr Madhu Vajpeyi HOD,Agrl. Biochem.
3 Dr Mukesh Mohan Prof , Agri Biochem
4 Dr Lallu Singh ProfAgri Biochem
5 Dr. Sarvendra Kumar Gupta HOD,GPB
6 Dr.D.P.Singh JDR
7 Dr.R.K. Pandey Asst.Prof.Office of DIR
8 Dr.S.P. Kushwaha Asst. Prof Crop Physiology
9 Dr.Nand Kumar Asst. Prof. Agrl. Biochem.
10 Er. P.K. Bisaria Asst. Prof. Agrl. Biochem.
11 Dr. Lokendra Singh EBL
12 Dr SB Pandey Assist Prof Soil Sci

Results of previous years experiments were displayed and discussed. New programs on biochemical aspects of some crops were discussed and finalized. All the programs were approved as such.

  • PROCEEDINGS OF RAC-KHARIF 2016-17

The RAC-Kharif 2016-17 of agricultural Biochemistry was held on 11/07/2016 in the department under the Chairmanship of Director Research .The following scientists participated in the meeting:

  • Dr. Har Gyan Prakash,Director Research
  • Dr.Madhu Vajpeyi,HOD ,ABC
  • Dr.D.R. Singh,Prof. Entomology
  • Dr.P.N.Yadav,Prof.SCWM
  • Dr.C.L.Maurya,HOD,Seed Technology
  • Dr.Ramchandra,HOD,Agrl. Economics & Statistics
  • Dr. R.K.Pandey,Asst.Prof. Directorate of Research
  • Dr. S.B. Pandey,Asst. Chemist,Soil Science & Agrl. Chemistry
  • Dr. Lallu Singh, Prof. Agrl. Biochemistry
  • Dr. Rakesh Babu, Asst. Prof. /Asst. Chemist-Legumes
  • Dr. Nand Kumar, Asst. Prof. /Rice Biochemist
  • Dr. S.P. Kushwaha, Asst. PErof.,Crop Physiology

There were three programmes. The following decisions were taken regarding the execution of experiments:

  • Experiment No. 1 was was approved with the remark that instead of early and late varieties ,it should be designated as short duration and long duration maturing varieties.The scientist was advised to contact persons at IIPR regarding maschine for dahl recovery for submitting a demand for its purchase to DIR.
  • Experiment No. 2 was was approved with the remark that number of samples for branded and unbranded samples should be same and should be of same variety e.g. Basmati branded and basmati unbranded.Insted of test weight it should be 1000 kernel weight
  • Experiment No. 3 was was approved as such.Iwas suggested that infuture varieties from other universities and hybrid varieties should also be tested.

Annual report of the department/performance of the department:

Annual Report 2012-13

Research

  • Evaluation of breeding material of rapeseed-mustard for oil content:
    Among rapeseed–mustard genotypes /varieties tested for oil content, the genotypes MK(E)-103,MK-11-107(>41%)in B.juncea, TK-9-2,TK-7-14(>44%)in B.rapa var.Toria and YSKM-11-202AND YSKM -11-107(>44%)were identified as high oil containing genotypes.
  • Evaluation of oil quality of rapeseed-mustard from Advance Varietal Trial:
    Among rapeseed –Mustard genotypes /varieties tested for fatty acid composition, Pusa mustard-21(1.81%) and LES-44(1.25%)were identified as low erucic material suitable for quality breeding as these contained less than 2% erucic acid in oil.
  • Anti-nutritional and antioxidative factors in seed meal of Brassica entries
    Out of 50 Brassica entries tested for Vitamin C and polyphenol content in their defatted seeds (meal), Vitamin C content was found to vary from 78.9 to134.6 mg/100g, the genotypes namely Bio -43, and Bio-45 recorded appreciably higher content of this anti-oxidant. As regards polyphenol content, it varied from 1.525 to 2.561%, the lowest and the highest values being given by the genotypes Bio- 37and Bio-27.
  • Processing techniques for better utilization of Bathua leaves:Investigations carried out to improve utilization of bathua through processing revealed that blanching of fresh bathua foliage in boiling water resulted in loss of total solid(2.83to 24.91%), nutritionally important microelements namely Zn(16.98to 43% ),Cu( 4.36to 38.09%) and Mn( 34.69 to 60.20%).
  • Biochemical evaluation of some varieties/hybrids of baby corn grown in kharif season:Among the varieties/hybrids of baby corn, hybrid R-2005-5 recorded highest amount of dry matter(9.82%),phosphorus(68mg/100g) and protein(1.05%) as compared to others.
  • Participation in seminar /symposia :Dr. Rakesh Babu, Asst. Prof. Participated in a National Seminar on “Emerging Trends in Input Management for Higher Agricultural Productivity”organized by Janta College,Bakewar,Etwah, from 26-27th, Feb.,2012.
  • Participation in workshop etc.:Dr Madhu Vajpeyi participated in 19th annual group meet of All India Coordinated Research Project on Rapeseed –Mustard, held at Birsa Agricultural University, Ranchi, Jharkhand, held in August,2012.

Annual Report 2013-14

Research

  • Evaluation of breeding material of rapeseed-mustard for oil content:
    Among rapeseed –mustard genotypes /varieties tested for oil content, the genotype RKE-9-1(43.07), RKE-8-2(42.89) in B.juncea, TKM-12-105 (45.29),TK- 9-1 (45.23), TK -9-3 (45.14), TKM-12-101 (44.96), TKM-12-106 (44.75) in B.rapa var.Toria and YSKM-12-106(45.36), YSKM-10-2(44.98)were identified as high oil containing genotypes.
  • Evaluation of oil quality of rapeseed-mustard from Advance Varietal Trial:
    Among rapeseed –Mustard genotypes /varieties tested for fatty acid composition, Pusa mustard -21,LES -43 and LES-45 containing 7.87, 4.05and2.75% erucic acid were identified as low erucic material suitable for quality breeding.
  • Anti-nutritional and antioxidative factors in seed meal of Brassica entries
    Out of 60 Brassica entries tested for Vitamin C and polyphenol content in their defatted seeds (meal), Vitamin C content was found to vary from 90 to 119.8 mg/100g, the genotypes namely Bio -46, 50 and 51 recorded appreciably higher content of this anti oxidant. As regards polyphenol content, it varied from 1.516 to 2.651%, the lowest and the highest values being given by the genotypes Bio-47 and Bio-6.
  • Blending mustard oil with other edible oils to identify the blend best suited for edible purpose:
    Oils and fats are essential nutrients which perform various functions in our body. None of the existing oils perfectly matches the WHO recommendations of a healthy cooking oil (SFA: MUFA: PUFA =1:1-3:1) This was only possible by blending judicious amount of the existing edible oils hence sesame and groundnut oils were chosen for blending with mustard oil. Among the oil-blends, mustard: sesame(30:70) and mustard: groundnut(30:70) proved to be the best from edible point of view whereas the oil of the blend mustard: groundnut(30:70) was found to be the most stable one as it showed the highest value of MUFA:PUFA
  • Effect of delayed sowing on biochemical composition of seeds of some mustard varieties/genotypes:
    In mustard, due to delay in sowing, from 15 Oct to 23 Nov, a decrease in 1000 seed weight and oil content was observed while protein content was found to increase. Sowing at optimum time recorded highest 1000 seed weight. Among varieties, highest 1000 seed weight was recorded by the genotype NRCDR -02 (4.57 g). Among the interactions, the genotype BPR-540-6 at first date of sowing (15 Oct), recorded highest 1000 seed weight (5.62 g) while at second date of sowing (23 Nov.) NRCDR-02 recorded highest test weight (4.11 g). Among the varieties, NPJ-113 recorded highest oil content, this genotype also performed the best in both the sowing dates. However NPJ-113 recorded lowest protein content among the varieties and also at first date of sowing.
  • Processing techniques for better utilization of Bathua leaves:
  • Variability in quality parameters of desi & Kabuli types chickpea varieties:The range of variability in Kabuli chickpea varieties were from 61.70-65.03% (Total carbohydrates), 23.38 – 25.90 % (Crude Protein), 4.12- 5.03% (Crude fat) and 373.48 – 407. 00 K cal/100g (calorific value) however in case of desi chickpea varieties, the rang of variability was as follow in total carbohydrates from 51.74- 65.05 % in crude protein from 23.68 – 25.57%, in crude fat from 3.62 – 4.62 % and in calorific value from 345.19 – 385.57 k cal/100g.Among the Kabuli & desi chickpea varieties tested, variety- BG 1053 (Kabuli type) appeared superior most from nutritional point of view while in case of desi chickpea varieties- Vishal & Sadabahar showed higher value of crude protein, calorific value, crude fat & total carbohydrates.
  • Effect of variance in dietary bulk nutrients and Ayurvedic/ Allopathic medicinal supplement on the blood glucose profiles of pre-diabetic /Type-2 diabetic subjects: During the study, it was found that a higher dietary protein and fibre go to reduce Post Prandial Blood Glucose (PPBG) level carbohydrates increased them. Among the allopathic medicines ‘Metformin’ reduced the mean value of fasting blood glucose (FBG) from 199 mg/dl to 130 mg/dl and PPBG form 270 mg/dl to 142 mg/dl on consuming one pill per day for a Type-2 diabetic person.
  • Participation in seminar /symposia :Dr.Rakesh Babu ,Asst.Prof.,Dr.Madhu Vajpeyi, Assoc. Prof. and Dr. Mukesh Mohan,Assoc. Prof., participated in National Seminar on Science for shaping the future of India, held on 3-4,Dec.,2012 at Mechant Chambers’ Hall,Kanpur, organised jointly by Indian Science Congress Association, Local Chapter & Dept. Of Zoology,D.A.V.College & D. G. College, Kanpur.
  • Participation in workshop etc.:Dr Madhu Vajpeyi participated in 20th annual group meet of All India Coordinated Research Project on Rapeseed –Mustard, held at S.K.Nagar Dantiwada Agricultural University,S.K.Nagar,Gujarat.

Annual Report 2014-15

Research

  • Evaluation of breeding material of rapeseed-mustard for oil content:Among rapeseed–mustard genotypes /varieties tested for oil content, the genotype TKM-13-108(43.21%),TKM-13-113(43.34%)inToria,KMR(L)-14-408and 409(>38%)in mustard and YSKM-13-104(41.43%)and YSKM-13-110(41.44%) in yellow sarson were identified as high oil containing genotypes.
  • Evaluation of oil quality of rapeseed-mustard from Advance Varietal Trial:
    Among rapeseed –Mustard genotypes /varieties tested for fatty acid composition, PDZ-4(0.02%),LES-51(0.21%),EJ8-369(0.11%),LES-50(0.24%),EJ8-118(0.28%),PDZ-3(0.18%),RLC-4(0%),PDZ-1(0.06%),PDZ-2(0.1%),RLC-3(0.14%),LES-49(0%),PM-30(00),and PM-29(00) were identified as low erucic material suitable for quality breeding.
  • Nutritional profiling of important breeding materials of ‘00’ genotypes of Rapeseed-Mustard from DRMR:
    Study of nutritional profile of important breeding materials of ‘00’ genotypes revealed a range of variation of 37.32 to 39.96%,0.62 to 0.89g/16 g N,0.42 to 0.59g/16 g N and 1.38 to 1.89% in protein, methionine ,tryptophan and lignin content,respectively wherein highest values were shown by BPRQ2-2-11(protein and methionine ) ,BPRQ2-1-5(tryptophan )and PDZ-2(lignin) .
  • To study the antioxidant activity in IVT/AVT quality breeding material:
    Among rapeseed –Mustard genotypes /varieties tested for antioxidant activity the genotypes EJ8-118(16.85mg/g AAE) , RLC-4(68.32%) Ej8-118(27.93%) were identified as promising ones as these recorded highest total antioxidant capacity, radical scavenging activity and metal chelating activity ,respectively.

Participation in workshop etc.:

  • Dr Madhu Vajpeyi participated in 22nd annual group meet of All India Coordinated Research Project on Rapeseed –Mustard, held at SIAM,Durgapura,Jaipur,Rajasthan from August3-5,2015.

Paper Published:

Abstract Published:

Rudranath,Madhu Vajpeyi and Lallu(2015) Biochemical constituents of mustard seeds as influenced by late sowing . Extended summary published in Proceedings of National Seminar on Strategic Interventions to Enhance Oilseed production. held at DRMR, Bharatpur from February 19-21,2015.

Major Extention achievement:

Organized a stall of Biochemistry .All the faculty members actively participated in kisan mela .

Annual Progress Report for the period 2015-16

Teaching

Ph.D. –

  • One student namely Mr. Rudra Nath,ID No. CA 7365/8was awarded Ph.D. Degree
  • One student namely Mr Deepak Kumar ID No. CA-18102/16, was admitted for Ph.D. degreeID No.

M.Sc.-

  • Two students namely Mr. Thombre Mahadeo Uttamrao I.D. No. CA9303/14 and Ms Alka Singh, I.D. No. CA 9299/14 were awarded M.Sc. degree.
  • Five  students namely Km. Priyanka,ID No. CA 9616/15, Sri Gangesh Kumar ShuklaID No. CA 9617/15, Sri Pramod KumarID No. CA 9618/15,  Km Deepika KannaujiaID No. CA 9619/15,Sri Sunder Lal, ID No. CA 9620/15,were admitted for M.Sc. degree .

U.G.  courses taught by faculty members

  • ABC-301 (Biochemistry) to B.Sc. (Hons) in Agriculture
  • ABC-301(Elementary Plant Biochemistry& Biotechnology ) to B.Sc. (Hons) in Horticulture
  • BSH-101(Plant Biochemistry& Biotechnology ) to B.Sc. (Hons) in Forestry
  • PHT-102(Fundamentals of Food Technology ) to B.Sc. (Hons) in Horticulture

Research 

Experiments performed & their results:

  • Biochemical evaluation of some wheat genotypes/varieties for chapatti making purpose: Biochemical analysis such as protein content, Gluten Content , Sedimentation value and Water Absorption in wheat flour were estimated last year . Results revealed that highest protein content was found in variety K-1006(13.45%) followed by K-402(13.32%),K-9107(13.29%)and K-9162(13.00%).Highest gluten content was recorded by the variety HD-2733(14.30%)followed by K-9162 and K-402.Highest sedimentation value was recoded by HD-2733(21.56(ml) whereas three varieties viz; K-607,K-9351 and K-9006 recorded highest water absorption (70%)during dough making.
  • Phsico-chemical investigation on certain genotypes/varieties of gram(Cicer aeritinum L.): Among the genotypes/varieties of gram highest dahl recovery of 87% was recorded in chickpea variety Pragati followed by Radhey.Pragati recorded lowest husk recovery of 11.42%.The variety Awarodhi showed lowest value of broken dhal % whereas lowest loss in processing was obtained iv variety KPG-59(1.76%).Results revealed that protein content among the chickpea genotypes varied from 21.00 to 24.10%. Highest protein content in dhal was recorded by the variety KWR-108.
  • Nutritional profiling of important quality breeding materials (DRMR):
    Nutritional constituents of seed viz;protein, methionine,tryptophan and phytic acid were estimated . a perusal of the data showed that protein content varied from 32.29% (PDZ-3)to 39.34(RLC-5),The other genotypes having >37% protein were: DRMRIJQ15-1 (38.95%), LES-53 (37.45%), RH 749 (ZC) (37.33%), PDZ -6 (37.84%), PDZ 1 (37.77%), Pusa mustard 21 (QC) (37.86%), PDZ 2 (38.67%), Kranti (NC) (37.56%).The range of variation in methionine was from 0.616(EJ 8-379)to 1.785 g/16g N (RLC 4 , EJ 8-118 ),the genotypes viz; PDZ 3 (1.692), NUDB-26-11 (1.455), Kranti (NC) (1.452), Kranti (NC) (1.398), RLC 3 (1.292), RLC 2 (LR) (1.244), Pusa mustard 21 (QC) (1.174), LES 50 (1.156)and PDZ-5 (1.116)had >1g/16g N methionine in them. Tryptophan content among the genotypes varied from 0.581 (RH 749 (ZC)) to 1.260 g/16g N (Kranti (NC)),the other promising genotypes were: RLC 2 (LR) (1.171 g/16g N) and PDZ 3 (1.035 g/16g N).
  • Evaluation of some Brassica (Rapeseed & Mustard) genotypes for seed oil and glucosinolate :Brassica genotypes /varieties were tested for seed colour ,seed size,test weight,oil content and glucosinolate. Seeds varied in colour in B.rapa variety toria(G1) from light brown to dark brown, in B.juncea variety yellow sarson(G2) only yellow colour and in B.juncea variety rai (G3)only dark brown colour was observed. All the entries in G1seeds were medium sized except T9 which had bold seeds,in G2 seed size ranged from small to bold .The entries YSKM-15-104, YSKM-15-106 and YSKM-15-109 had bold seeds while in G3 seed size ranged from small to medium.KMR-15-103, KMR-15-104, KMR-15-107, KMR-15-109 and KMR-15-110 were bold seeded. The mean 1000 seed weight of toria (3.77g)group was lowest whereas maximum mean 1000 seed weight was noticed in yellow sarson group(4.20g).Oilcontent in seeds was found to vary from 35 to 42 % in G1 and G2 groups and from 33 to 42% in G3 group. In toria TKM -15-103 and TKM-15-107showed highest oil content(42%)and Glucosinolate in moderate and trace amount,respectively. In yellow sarson group , YSKM -15-104recorded 41% oil with moderately high glucosinolate.In rai group, KMR-15-103 and KMR-15-109 displayed highest oil content(42%)with moderately high glucosinolate .
  • Scientist attended  workshop :
S.No. Name ,Designation Workshop Place Date
1. Dr Madhu Vajpeyi,Professor /Biochemist 23rd Annual group meet of All India Coordinated Research Project on Rapeseed –Mustard DUVASU,Mathura August5-7,2016

Paper Published:

Rudranath,Madhu Vajpeyi and Lallu.Oil content and oil quality of seeds of some mustard [Brassica juncea (L.) Czern & Coss] genotypes as affected by late sowing.
Paper accepted for publication in Indian J. Agric. Biochem.2016.vol 1.

Extension

  • Faculty members actively participated in Kisan Mela Organized by the university.
  • A technique for making ‘Hara Panir’’ a protein isolate prepared from green leaves was demonstrated.

Tender and quotations related information:

Governed by SPO of the university